This study reports the enrichment nd isolation of a microbial culturew capable of degrading endosulfan with minimal production of endosulfan sulfate, the toxic metabolite of endosulfan, from tropical acid soil. The results of this study suggest that this novel strain is a valuable source of potent endosulfan- degrading enzymes for use in enzymatic bioremedation. This clearly states that endosulfan does not bio- accumulate due to the microbial culture in the soil.