Free Essays on Enzyme to Get Inspired & Learn by Example

Introduction

Alkaline phosphatase is an enzyme that is most critical in the process of biomineralization (Whitehurst & Law 2002). The enzyme enhances the hydrolysis of substances that constitute phosphates, generates orthophosphate besides enhancing the calcium uptake. Alkaline phosphatase is a hydrolytic enzyme responsible for removing phosphate groups from DNA and proteins. A typical substrate for the enzyme is para-nitrophenol phosphate that is hydrolysed to para nitrophenol by the enzyme. This has an absorbance at 410nm and so activity of the enzyme can be measured spectrophotometrically.
In humans, the main sources of alkaline phosphatase are the liver, bile duct, kidney bone and Continue reading...

Objective: Investigate the effect of increasing temperature on enzyme activity.

Introduction: Enzymes are biological catalyst. All most all the functions in the cell are regulated by these enzymes. Except for RNAase, all most all the enzymes are proteins. Enzymes are very efficient and specific in the reaction they catalyze. All enzymes exhibit four common features: I. Enzymes do not make a reaction to occur; they can only fasten the reaction rate. II. Enzyme molecule is not permanently altered by the reaction, though they may be altered transiently. However at the end of the reaction they emerged unchanged and can be used over and over again. III. Enzyme can catalyze Continue reading...

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Aim:

The objective of this report is to review the steps that were conducted in the PCR directed RFLP evaluation.
Evidence Review
The fundamental technique for the detection of the restriction fragmented length polymorphism entails the segmentation of the DNA sample by applying a restriction enzyme. The process applies the differential in the sequences of the homologous DNA that can be found by the presence of DNA segments subsequent to their absorption. The restriction fragmented length polymorphism probes are applied in the evaluation of variations.

First Extraction

The complete DNA is absorbed with an enzyme that Continue reading...

Introduction

Niemann-Pick Disease represents a group of inherited, genetic disorders, clinically referred to as leukodystrophies, which are more commonly known as lipid storage disorders. In each of the variations of the conditions all share one specific aspect in common, they cause lipids to not be properly metabolizes and inappropriately allows lipids, fats, to accumulate in different organs of the body, including the liver and brain. Over time this accumulations become severe enough that permanent damaged is caused (Kugler, 2014). This condition, due to its genetic origins, is inherited and is often a cause of serious health problems in new infants, Continue reading...

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This lab experiment is aimed at studying the rate of reaction involving enzymes by observing the change in absorbance when the reaction is carried out. This experiment will illustrate both the methods of using the linked assay method.

Introduction

The reaction rate of reactions involving enzymes is studied spectrophotometrically by monitoring the change in absorbance at different intervals during the reaction. The accurate measurement of glucose is important in the diagnosis and management of hyperglycemia and hypoglycemia. The enzymes that will be used in this experiment are Hexokinase (HK) and Glucose-6-Phosphate Dehydrogenase (G6PDH). The nicotinamide coenzymes are Nicotinamide Continue reading...

The technological advancement has introduced numerous achievements in the field of biotechnology. Microbial transformation or biotransformation is the phenomenon where living organisms are used in modifying materials that are not frequently used for growth (Donova and Egorova). Apart from animal cells, plant tissues and enzymes, microbial transformation is associated with the reactions under biotransformation. A substance can be transformed into another product through microbial action on the substrate. Studies reported that the utilization of biotechnology in the formulation of steroids was introduced in the year 1952. Murray and Peterson developed 1 lα-hydroxylation using Rhizopus and utilized it effectively Continue reading...

Sickle cell anemia (SCA) is the form sickle cell disease (SCD) that is most widely known. In this condition, the body makes red blood cells that are sickle-shaped. Sickle cells have abnormal hemoglobin which causes them to have a sickle-shape. These cells are sticky and stiff, blocking blood vessels, which damages organs and causes pain. This discussion will be based on the genetic predisposition of sickle cell anemia as well as the biochemical processes that occur in the normal and diseased condition.

Different processes in the normal and diseased state

Linus Pauling and associates in 1949 described sickle cell anemia (SCA) as the first “molecular disease” coining Continue reading...

Introduction: Niacinamide is an amide of niacin. It is the active form of the vitamin. Niacin is converted to niacinamide predominantly in liver. Other body tissues are also capable to amide conversion. Both the forms of vitamin are heterocyclic aromatic compound. They are collectively called Vitamin B3. Yeast, milk, fish, eggs, green vegetables, beans and cereal grains are rich sources of this vitamin. People whose diet is solely based on staple food like corn, are likely to suffer from niacin deficiency. Tryptophan is a substrate for in vivo synthesis of niacin, and this amino acid is deficient in corn. Continue reading...

Staphylococci

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Foodborne diseases are common in all parts of the world. Foodborne diseases are associated with the food consumed. These diseases can be categorized into two forms based on the cause of the disease. Food intoxication or food poisoning and food infection are the two divisions of the foodborne diseases. Food intoxication is the result of consumption of toxins along with the food. Toxins can be produced by the bacteria, fungi, or they may be already present in the food, for instance, some mushrooms are toxic. These mushrooms Continue reading...

Abstract

Growth incorporates a uniform increase in the volume of cellular components. All organisms grow as a main biological characteristics. Growth is a factor that can be measured using a number of parameters and determined standards incorporated as well. The reproduction amongst most bacteria take place through binary fission. There are factors affecting growth amongst organisms such as temperature. Since growth depends largely on enzyme activity, which also relies greatly on temperature then the former can be used as a factor of the latter and both can provide reliable information in determining the population growth. Throughout this laboratory evaluation, rates Continue reading...