Introduction
Polyacrylamide gel electrophoresis is a fundamental technique applicable in most biological investigations ranging from biochemistry to forensic. The mechanism is used for the separation of macromolecules especially proteins together with nucleic acids with respect to the electrophoretic mobility of the molecules being separated.
Electrophoresis is a relatively simple method to study the properties of proteins. It is based on the principle that charged molecules will migrate through a matrix when an electric field is applied. The matrix used is polyacrylamide. NB Polyacrylamide is highly toxic, carcinogenic and teratogenic. The chemical agents used to form the polyacrylamide matrix are acrylamide
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