Immunoprecipitation of Activated MAP Kinase
MAP Kinases are protein kinases which have many roles in different biological processes. MAP Kinase ERK1/2 can be phosphorylated by another protein kinase at two amino acid residues and is involved in phosphorylating other target proteins. The following laboratory experiment examines the phosphorylation of MAP Kinase ERK1/2 from rat muscle cells under stimulating (in the presence of insulin) and inhibitory (in the presence of PD98059) conditions. Phosphorylated forms of MAP Kinase were detected by immunoprecipitation using an antibody that is specific for phosphotyrosine, followed by immunoblot analysis. The results of this experiment are discussed.
MAP Kinases, also known Continue reading...