This lab experiment is aimed at studying the rate of reaction involving enzymes by observing the change in absorbance when the reaction is carried out. This experiment will illustrate both the methods of using the linked assay method.
The reaction rate of reactions involving enzymes is studied spectrophotometrically by monitoring the change in absorbance at different intervals during the reaction. The accurate measurement of glucose is important in the diagnosis and management of hyperglycemia and hypoglycemia. The enzymes that will be used in this experiment are Hexokinase (HK) and Glucose-6-Phosphate Dehydrogenase (G6PDH). The nicotinamide coenzymes are Nicotinamide Continue reading...