Literature Review On High Pressure Liquid Chromatography For Identification Of Haemoglobin Variants
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Key Words: High Pressure Liquid Chromatography, HPLC, Haemoglobin, Haemoglobinopathy, Electrophoresis, Thalassaemia.
Thesis Topic: High Pressure Liquid Chromatography for Identification of Haemoglobin Variants
Definition of High pressure liquid chromatography (HPLC)
Significance of the Study
Definition of terms like haemoglobin, types of haemoglobin variants, electrophoresis.
BODY:(4 TO 8)
Various haemoglobin variants
Existing methods for detection of haemoglobin variants
Necessity for its detection
Studies already conducted and included from databases like EBSCO, CINAHL, MEDLINE, Pro Quest.
PubMed based articles and scientific research papers
International guidelines and recommendations like Thalassemia International Foundation, haematology and haemato-oncology boards and associations.
Incorporation of molecular techniques and their pros and cons.
Conclusion of the study.
Best methodology in light of studies and various reviews
Recommendation by various researchers and influential and renowned authorities regarding use of a particular testing modality based on its efficacy and sensitivity.
REFERENCES AND WORKS CITATION.(10)
George, E., Jamal, A., Khalid, F., & Osman, K. (2001). High Performance Liquid Chromatography (HPLC) As A Screening Tool For Clasical Beta-Thalassaemia Trait In Malaysia. Malaysian Journal of Medical Sciences, 8(2), 40-46.
Gosselin, R. C., Carlin, A. C., & Dwyre, D. M. (2011). Comparison of the BioRad Variant and Primus Ultra2 high-pressure liquid chromatography (HPLC) instruments for the detection of variant hemoglobins. International Journal Of Laboratory Hematology, 33(2), 159-167. doi:10.1111/j.1751-553X.2010.01260.x
Harteveld, C., Synodinos, J., Petrou, M., Angastiniotis, M., & Galanello, R. (2012). Haemoglobin Pattern Analysis. In J. Old (Ed.), Prevention of Thalassaemias and Other Haemoglobin Disorders (2nd ed., Vol. 2, p. 35). Nicosia, Cyprus: Thalasaemia International Federation.
Khalil, M. S., Molyneux, A. T., Marouf, S., Eldamanhory, G. A., Schuh, A. H., Henderson, S. J., & Old, J. M. (2009). The accurate prediction of rare hemoglobin variants using a combination of high performance liquid chromatography, retention time and isoelectric focusing electrophoresis position. Saudi Medical Journal, 30(9), 1158-1164.
Lippi, G, et al. (2007). Separation Of Haemoglobin Hbe And Hba2 By The Fully Automated, High-Pressure Liquid Chromatography Tosoh HLC-723 G7 Analyzer. International Journal Of Laboratory Hematology, 30, 432-436.
Moorchung N, Phillip J, Sarkar RS, Prasad R, Dutta V. Is high pressure liquid chromatography an effective screening tool for characterization of molecular defects in hemoglobinopathies?. Indian Journal of Pathology and Microbiology 2013;56:36-9
Pornprasert, S., Kasemrad, C., & Sukunthamala, K. (2010). Diagnosis of Thalassemia on Dried Blood Spot Samples by High Performance Liquid Chromatography. Hemoglobin,34(5), 486-494.
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